Publications

T. Menegatti, Ž. Lavrič, P. Hlebanja, P. Žnidaršič-Plazl. Microfluidics-based generation and immobilization of nanoscale cross-linked enzyme aggregates for continuous transamination. Chem. Eng. J. 2025, 523, 168865168865.

M. Božinović, M. Jereb, B. Šketa, A. Gaber, M. Seručnik, J. Košmrlj, P. Žnidaršič-Plazl. Development of sustainable biocatalytic furfurylamine production in a magnetic field-assisted microfluidic reactor. ACS Sustain. Chem. Eng. 2025, 13 (38), 15887-15896.

H. Potočnik, Y. Šmigoc, I. Plazl, P. Žnidaršič-Plazl, R. Ambrožič. Model-based design of a microfluidic pervaporation device for intensified VOC separation. Sep. Purif. Technol. 2025, 378 (3), 134795.

T. Kopač, R. Ambrožič. Tailored alginate and chitosan hydrogels: structural control and functional applications via copper-based electrodeposition. Int. J. Biol. Macromol. 2025, 308 (1), 142476.

Y. Hen, T. Zhu, C. Qi, I. Plazl, P. Žnidaršič-Plazl, K. Zhao, Y. Fu, Y. Miao, Y. Gao, F. Wang. Plasma-assisted chemical-looping dry reforming of methane for hydrogen generation at room temperature. Fuel, 2025, 400, 135664.

N. Allasia, O. Nevskyi, M. Marelli, I. Plazl, J. Albertazzi, V. Busini, F. Castiglione, F. Rossi, G. Vilé. Bijel-based mesophotoreactor with integrated carbon nitride for continuous-flow photocatalysis. Chem. Eng. J. 2024, 499, 155885.

M. Logarušić, K. Šubar, M. Nikolić, A. Jurinjak Tušek, A. Damjanović, M. Radović, I. Radojčić Redovniković, P. Žnidaršič-Plazl, W. Kroutil, M. Cvjetko Bubalo. Harnessing the potential of deep eutectic solvents in biocatalysis: design strategies using CO2 to formate reduction as a case study. Front. Chem. 2024, 12, 1467810.

M.A. Asgharpour, J. Pantakitcharoenkul, M. Coblyn, I. Plazl, G.N. Jovanovic. Time scale analysis of enzymatic reduction of uric acid in a microfluidic biomedical device. Front. Chem. Eng. 2024, 6, 1451222.

A. Ramšak, M. Gazvoda, I. Plazl, R. Ambrožič. Cu-alginate hydrogels in microfluidic systems: a sustainable catalytic approach for click chemistry. Front. Chem. Eng. 2024, 6, 1434131.

An interview with Prof. Polona Žnidaršič Plazl. Chem. Eng. Process. 2024, 205, 109909.

M. Seručnik, I. Dogsa, L.J. Zadravec, I. Mandic-Mulec, P. Žnidaršič-Plazl. Development of a microbioreactor for Bacillus subtilis biofilm cultivation. Micromachines, 2024, 15 (8), 1037.

B. Šketa, J. L. Galman, N. J. Turner, P. Žnidaršič-Plazl. Immobilization of His6-tagged amine transaminases in microreactors using functionalized nonwoven nanofiber membranes. N. Biotechnol. 2024, 83, 46–55.

T. Menegatti, T. Kopač, P. Žnidaršič-Plazl. Tuning mechanical characteristics and permeability of alginate hydrogel by polyvinyl alcohol and deep eutectic solvent addition. Bioengineering, 2024, 11 (4), 371.

R. Ambrožič, U. Krühne, I. Plazl. Microfluidics with redox-responsive hydrogels for on-demand BPA degradation. Chem. Eng. J., 2024, 485, 149542.

T. Menegatti, I. Plazl, P. Žnidaršič-Plazl. Model-based design of continuous biotransformation in a microscale bioreactor with yeast cells immobilized in a hydrogel film. Chem. Eng. J., 2024, 483, 149317.

T. Menegatti, Ž. Lavrič, P. Žnidaršič-Plazl. Microfluidics-based preparation of cross-linked enzyme aggregates. WO2023175002A1.

P. F. Bergamo Dias Martins, I. Plazl, D. Strmcnik, B. Genorio. Prospect of microfluidic devices for on-site electrochemical production of hydrogen peroxide. Curr. Opin. Electrochem. 2023, 38, 101223.

G. Koplányi, E. Bell, Z. Molnár, G. Katona, P. L. Neumann, F. Ender, G. T. Balogh, P. Žnidaršič-Plazl, L. Poppe, D. Balogh-Weiser. Novel approach for isolation and immobilization of a recombinant transaminase applying an advanced nanocomposite system. ChemBioChem 2023, e202200713.

A. Zeko-Pivać, M. Tišma, P. Žnidaršič-Plazl, B. Kulisic, G. Sakellaris, J. Hao, M. Planinić. The potential of brewer's spent grain in the circular bioeconomy: state of the art and future perspectives. Front. Bioeng. Biotechnol., 2022, 10, 1-15.

M. Ružič, J. Svetelj, S. Zupančič, I. Plazl, P. Žnidaršič Plazl, M. Bitenc, B. Regina. A continuous process for the preparation of (S)-methyl N((2′-cyano-[1,1′-biphenyl]-4-yl)methyl)-N-pentanoylvalinate in a flow reactor. European patent application EP 3 939 967 A1, 2022-01-19. München: European Patent Office.

R. Ambrožič, I. Plazl. Development of an electrically responsive hydrogel for programmable in situ immobilization within a microfluidic device. Soft Matter, 2021, 17, 6751–6764.

T. Menegatti, P. Žnidaršič-Plazl. Hydrogel-based enzyme and cofactor co-immobilization for efficient continuous transamination in a microbioreactor. Front. Bioeng. Biotechnol., 2021, 9, 752064.

P. Žnidaršič-Plazl. Biocatalytic process intensification via efficient biocatalyst immobilization, miniaturization, and process integration. Curr. Opin. Green Sustain. Chem., 2021, 32, 100546.

M. Tišma, P. Žnidaršič-Plazl, G. Šelo, I. Tolj, M. Šperanda, A. Bucić-Kojić, M. Planinić. Trametes versicolor in lignocellulose-based bioeconomy: state of the art, challenges and opportunities. Bioresour. Technol., 2021, 330, 124997.

G.N. Jovanovic, M. Coblyn, I. Plazl. Time scale analysis & characteristic times in microscale-based chemical and biochemical processes. Part 1, Concepts and origins. Chem. Eng. Sci., 2021, 238, 116502.

G.N. Jovanovic, M. Coblyn, I. Plazl. Time scale analysis & characteristic times in microscale-based chemical and biochemical processes. Part 2, Bioreactors with immobilized cells, and process flowsheet analysis. Chem. Eng. Sci., 2021, 236, 116499.

F. Strniša, T. V. Sagar, P. Djinović, A. Pintar, I. Plazl. Ni-containing CeO2 rods for dry reforming of methane: activity tests and a multiscale lattice Boltzmann model analysis in two model geometries. Chem. Eng. J., 2021, 413, 127498.

D.K. Martin, O. Vicente, T. Beccari, M. Kellermayer, M. Koller, R. Lal, R.S. Marks, I. Marova, A. Mechler, D. Tapaloaga, P. Žnidaršič-Plazl, M. Dundar. A brief overview of global biotechnology. Biotechnol. Biotechnol. Equip., 2021, 35 (1), 354-363.

P. Žnidaršič-Plazl. Let the Biocatalyst Flow. Acta Chim Slov., 2021, 68, 1-16.

A. Hubman, I. Plazl, T. Urbič. Inertial focusing of neutrally buoyant particles in heterogenous suspensions. J. Mol. Liq., 2021, 328, 115410.

M. Seručnik, F. A. Vicente, Ž. Brečko, J.A.P. Coutinho, S.P.M. Ventura, P. Žnidaršič-Plazl. Development of a Microfluidic Platform for R‑Phycoerythrin Purification Using an Aqueous Micellar Two-Phase System. ACS Sustainable Chem. Eng., 2020, 8 (46), 17097-17105.

F. Strniša, P. Žnidaršič-Plazl, I. Plazl. Lattice Boltzmann modeling-based design of a membrane-free liquid-liquid microseparator. Chem. Biochem. Eng. Q., 2020, 34, 73-78.

F. A. Vicente, I. Plazl, S.P.M. Ventura, P. Žnidaršič-Plazl. Separation and purification of biomacromolecules based on microfluidics. Green Chem., 2020, 22, 4391-4410.

F. Strniša, T. Urbič, I. Plazl. A lattice Boltzmann study of 2D steady and unsteady flows around a confined cylinder. J. Braz. Soc. Mech. Sci. & Eng., 2020, 42 (2), 1-13.

G. Stojkovič, P. Žnidaršič-Plazl. Covalent Immobilization of Microbial Cells on Microchannel Surfaces. In: Guisan J., Bolivar J., López-Gallego F., Rocha-Martín J. (Eds.) Immobilization of Enzymes and Cells. Methods in Molecular Biology, vol 2100. Humana Press, New York, NY, 2020, pp. 417-426.

T. Menegatti, P. Žnidaršič-Plazl. Copolymeric hydrogel-based immobilization of yeast cells for continuous biotransformation of fumaric acid in a microreactor. Micromachines, 2019, 10, 867.

P. Žnidaršič-Plazl. The promises and the challenges of biotransformations in micro-flow. Biotechnol. J., 2019, 14, 1800580.

F. Strniša, T. Urbič, P. Žnidaršič-Plazl, I. Plazl. Process intensification and miniaturization of chemical and biochemical processes. Comput. Aided Chem. Eng., 2019, 1801-1806.

F. Strniša, M. Bajić, P. Panjan, I. Plazl, A.M. Sesay, P. Žnidaršič-Plazl. Characterization of an enzymatic packed-bed microreactor: Experiments and modeling. Chem. Eng. J., 2018, 350, 541-550.

N. Miložič, G. Stojkovič, A. Vogel, D. Bouwes, P. Žnidaršič-Plazl. Development of microreactors with surface-immobilized biocatalysts for continuous transamination. N. Biotechnol., 2018, 47, 18-24.

M. Bajić, P. Žnidaršič-Plazl, M. Kingston, V. Hessel. General aspects of immobilized biocatalysts and their applications in flow. In: Science of Synthesis: Knowledge Updates 2018/1. Stuttgart; New York: Georg Thieme. 2018, pp. 397-443.

P. Žnidaršič-Plazl, I. Plazl. Microbioreactors. In: Comprehensive Biotechnology, Vol. 2, Engineering perspectives in biotechnology. 3rd Ed. Elsevier, 2017, pp 414-427.

P. Žnidaršič-Plazl. Biotransformations in microflow systems: Bridging the gap between academia and industry. J Flow Chem., 2017, 7 (3-4), 111-117.

N. Miložič, M. Lubej, M. Lakner, P. Žnidaršič-Plazl, I. Plazl. Theoretical and experimental study of enzyme kinetics in a microreactor system with surface-immobilized biocatalyst. Chem. Eng. J., 2017, 313, 374-381.

M. Bajić, I. Plazl, R. Stloukal, P. Žnidaršič-Plazl. Development of a miniaturized packed bed reactor with ω-transaminase immobilized in LentiKats®. Process Biochem., 2017, 52, 63-72.

A. Prašnikar, T. Urbič, I. Plazl. Microscale technology and biocatalytic processes. Part 1, How to minimize the gap between research and industry. Chim. Oggi – Chem. Today, 2017, 35 (3), 18-20.

A. Prašnikar, T. Urbič, I. Plazl. Microscale technology and biocatalytic processes. Part 2, Scale-up case of surface enzyme-catalyzed biotransformation. Chim. Oggi – Chem. Today, 2017, 35 (4), 12-14.

U. Novak, D. Lavric, P. Žnidaršič-Plazl. Continuous lipase B-catalyzed isoamyl acetate synthesis in a two-liquid phase system using Corning® AFR TM module coupled with a membrane separator enabling biocatalyst recycle. J. Flow Chem., 2016, 6, 33-38.

P. Žnidaršič-Plazl, B. Zelić. Implementation of microreactor technology in biotechnology: IMTB 2015 conference (editorial). J. Flow Chem., 2016, 6, 1-2.

I. Pribec, T. Urbič, I. Plazl. Modelling reaction-diffusion dynamics in microsystems. In: 26th European Symposium on Computer Aided Process Engineering. Elsevier, 2016, 38, 1623-1628.

R. Wohlgemuth, I. Plazl, P. Žnidaršič-Plazl, K. V. Gernaey, J. M. Woodley. Microscale technology and biocatalytic processes: opportunities and challenges for synthesis. Trends Biotechnol., 2015, 33, 302-314.

G.N. Jovanovic, J. E. Atwater, P. Žnidaršič-Plazl, I. Plazl. Dechlorination of Polychlorinated Phenols on Bimetallic Pd/Fe Catalyst in a Magnetically Stabilized Fluidized Bed. Chem. Eng. J., 2015, 274, 50-60.

M. Lubej, U. Novak, M. Liu, M. Martelanc, M. Franko, I. Plazl. Microfluidic droplet-based liquid-liquid extraction: online model validation. Lab Chip, 2015, 15, 2233-2239.

U. Novak, M. Lakner, I. Plazl, P. Žnidaršič-Plazl. Experimental studies and modeling of α-amylase aqueous two-phase extraction within a microfluidic device. Microfluid. Nanofluid., 2015, 19, 75-83.

G. Stojkovič, M. Krivec, A. Vesel, M. Marinšek, P. Žnidaršič-Plazl. Surface cell immobilization within perfluoroalkoxy microchannels. Appl. Surf. Sci., 2014, 320, 810-817.

P. Žnidaršič-Plazl, B. Zelić. Implementation of Microreactor Technology in Biotechnology IMTB Conference: a new platform for an interdisciplinary science and technology: editorial. Chem. Biochem. Eng. Q., 2014, 28, 1-2.

N. Miložič, M. Lubej, U. Novak, P. Žnidaršič-Plazl, I. Plazl. Evaluation of diffusion coefficient determination using a microfluidic device. Chem. Biochem. Eng. Q., 2014, 28, 215-223.

M. Lubej, I. Plazl. Theoretical and experimental study of iron catalyst preparation by chemical vapor deposition of ferrocene in air. Chem. Eng. J., 2014, 242, 306-312.

P. Žnidaršič-Plazl. Enzymatic microreactors utilizing non-aqueous media. Chimica Oggi – Chem. Today, 2014, 32, 54-61.

B. Skubic, M. Lakner, I. Plazl. Sintering behavior of expanded perlite thermal insulation board: modeling and experiments. Ind. Eng. Chem. Res., 2013, 52, 10244-10249.

M. Ilu, U. Novak, I. Plazl, M. Franko. Optimization of a thermal lens microscope for detection in a microfluidic chip. Int. J. Thermophysics, 2013.

P. Žnidaršič-Plazl, B. Zelić. Implementation of Microreactor Technology in Biotechnology (IMTB) 2013 Conference: a new community for new perspectives. (editorial) Green Proc. Synth, 2013, 2, 537-539.

B. Ungerböck, A. Pohar, T. Mayr, I. Plazl. Online oxygen measurements inside a microreactor with modeling of transport phenomena. Microfluid Nanofluid., 2013, 14, 565-574.

U. Novak, P. Žnidaršič-Plazl. Integrated lipase-catalyzed isoamyl acetate synthesis in a miniaturized system with enzyme and ionic liquid recycle. Green Proc Synth., 2013, 2, 561-568.

M. Cvjetko Bubalo, I. Sabotin, I. Radoš, J. Valentičič, T. Bosiljkov, M. Brnčić, P. Žnidaršič-Plazl. A comparative study of ultrasound-, microwave-, and microreactor-assisted imidazolium-based ionic liquid synthesis. Green Proc. Synth., 2013, 2, 579-590.

M. Cvjetko, J. Vorkapić-Furač, P. Žnidaršič-Plazl. Isoamyl acetate synthesis in imidazolium-based ionic liquids using packed bed enzyme microreactor. Process Biochem., 2012, 47, 1344-1350.

G. Stojkovič, P. Žnidaršič-Plazl. Continuous synthesis of L-malic acid using whole-cell microreactor. Process Biochem., 2012, 47, 1102-1107.

U. Novak, A. Pohar, I. Plazl, P. Žnidaršič-Plazl. Ionic liquid-based aqueous two-phase extraction within a microchannel system. Sep. Purif. Technol., 2012, 97, 172–178.

A. Pohar, M. Lakner, I. Plazl. Parallel flow of immiscible liquids in a microreactor: modeling and experimental study. Microfluid. Nanofluid., 2012, 12, 307-316.

M.P.C. Marques, P. Fernandes, J.M.S. Cabral, P. Žnidaršič-Plazl, I. Plazl. Continuous steroid biotransformations in microchannel reactors. New Biotechnol., 2012, 29, 227-234.

A. Pohar, P. Žnidaršič-Plazl, I. Plazl. Integrated system of a microbioreactor and a miniaturized continuous separator for enzyme catalyzed reactions. Chem. Eng. J., 2012, 189–190, 376–382.

P. Žnidaršič-Plazl, I. Plazl. Microbiorectors. In: Comprehensive Biotechnology, 2nd Ed., Amsterdam: Elsevier, 2011, pp. 289-301.

G. Stojkovič, I. Plazl, P. Žnidaršič-Plazl. L-Malic acid production within a microreactor with surface immobilised fumarase. Microfluid. Nanofluid., 2011, 10, 627-635.

M. Cvjetko, P. Žnidaršič-Plazl. Ionic liquids within microfluidic devices. In: Ionic liquids: theory, properties, new approaches. Intech, 2011, 681-700.

I. Plazl, M. Lakner. Modeling and finite difference numerical analysis of reaction-diffusion dynamics in a microreactor. Acta Chim. Slov, 2010, 57, 100-109.

M. Tišma, P. Žnidaršič-Plazl, I. Plazl, Đ. Vasić-Rački, B. Zelić. Oxidation of coniferyl alcohol catalyzed by laccases from Trametes versicolor. Acta Chim. Slov., 2010, 57, 110-117.

P. Žnidaršič-Plazl, I. Plazl. Development of a continuous steroid biotransformation process and product extraction within microchannel system. Catal. Today, 2010, 157, 315-320.

M.P.C. Marques, P. Fernandes, J.M.S. Cabral, P. Žnidaršič-Plazl, I. Plazl. On the feasibility of in-situ steroid biotransformation and product recovery in microchannels. Chem. Eng. J., 2010, 160, 708-714.

G. Stojkovič, P. Žnidaršič-Plazl. Immobilization of yeast cells within microchannels of different materials. Acta Chim. Slov., 2010, 57, 144-149.

P. Žnidaršič-Plazl. Mikroreaktorska tehnologija: še ena prelomnica v znanosti in tehnologiji. Delo (Ljubl.), 18. 11. 2010, 52: 24.

A. Pohar, I. Plazl. Process intensification through microreactor application. Chem. Biochem. Eng. Q., 2009, 23, 537-544.

M. Tišma, B. Zelić, Đ. Vasić-Rački, P. Žnidaršič-Plazl, I. Plazl. Modelling of laccase-catalyzed L-DOPA oxidation in a microreactor. Chem. Eng. J., 2009, 149, 383-388.

A. Pohar, I. Plazl, P. Žnidaršič-Plazl. Lipase-catalyzed synthesis of isoamyl acetate in an ionic liquid/n-heptane two-phase system at the microreactor scale. Lab Chip, 2009, 9, 3385-3390.

P. Žnidaršič-Plazl, I. Plazl. Modelling and experimental studies on lipase-catalyzed isoamyl acetate synthesis in a microreactor. Process Biochem., 2009, 44, 1115-1121.

M. Lakner, I. Plazl. The finite differences method for solving systems on irregular shapes. Comput. Chem. Eng., 2008, 32, 2891-2896.

A. Pohar, I. Plazl. Laminar to turbulent transition and heat transfer in a microreactor: mathematical modeling and experiments. Ind. Eng. Chem. Res., 2008, 47, 7447-7455.

P. Žnidaršič-Plazl, I. Plazl. Steroid extraction in a microchannel system: mathematical modelling and experiments. Lab Chip, 2007, 7, 883-889.

G. Jovanović, P. Žnidaršič-Plazl, P. Sakrittichai, K. Al-Khaldi. Dechlorination of p-chlorophenol in a microreactor with bimetallic Pd/Fe catalyst. Ind. Eng. Chem. Res. 2005, 44, 5099-5106.